Cytometry core facility | Instrument information and flow cytometry software

Analysers

LSR Fortessa (Becton Dickinson)

fortressa

Gallios (Beckman Coulter)

gallios

Northern Lights 3000 CLC (Cytek Biosciences)

NorthernLights

Cytoflex S (Beckman Coulter)

Cytoflex

Sorting

Session times: 9:30 - 5:00, or by appointment.
Allow for 30min machine setup + 25 min per sample when booking.

MoFlo Astrios (Beckman Coulter)

astrios

Microscopes

LSM 700 (Zeiss)

lsm

LSM 800 (Zeiss) airyscan

lsm 800

Super resolution SP8 STED (Leica)

SP8

LSM 710 (Zeiss)

2-photon

Flow cytometry analysis software

Software dongles are available for loan. The dongles can be found on level 9.

Please do not hold these dongles overnight unless confirmed with a Detmold Staff member that the dongle is not required for installation or training in the morning. Please do not take these dongles off site, i.e. they are never to be taken home for use. If the dongle is lost the cost of replacing the software licence will be billed according to its loss.

A fair use policy applies to all of the software dongles. Please be aware that Detmold Staff may contact you for return of the dongle if your usage has exceeded a fair limit.

FCS express v7 New	Internet licence. Windows 7/8/10/11; macOS 10.12+. Can be used for offline analysis of flow data from any analyser or sorter. Cytoflex data needs manual adjustment (see cytoflex on the instruments page). Note: does not have cell cycle or proliferation modules.
FCS express v6	Older version of FCS express. Works on windows 7/8/10/11; macOS upto 10.14. Can analyse data from any sorter or analyser. Cytoflex data needs manual adjustment (see cytoflex on the instruments page). This licence includes the modules for cell cycle analysis and proliferation analysis.
FCS express v4	Older version of FCS express (replaced by v6 in Jan 2017). Does not have access to additional modules.
FCAP Array	Software for analysis of Cytometric Bead Arrays (CBA) from BD
Kaluza	Software package released by Beckman Coulter for offline analysis
FlowJo	Licences can be purchased from SAHMRI.

User category	CCB Staff	External academic	Commercial
Cell sorting	$60/hr	$100/hr	$200/hr
Northern Lights	$42.5/hr	$42.5/hr	$120/hr
Fortessa	$30/hr	$50/hr	$120/hr
Gallios / Cytoflex	$20/hr	$35/hr	$70/hr
Operator-fee	$30/hr	$40/hr	in-price
Sort tubes	$7.95 per bag of 25
Analysis tubes	$15.05 per bag of 500
Analysis-tube lids	$20.75 (1000pc) $8.67 (250pc)
non-sterile filter BD 30 µm	$2.6 each (with 1mL syringe)

Confocals	$28/hr	$60/hr	$240/hr
STED	$28/hr	$50/hr	$240/hr

Channel	Optical filter	Fluorochromes (405nm/488nm/640nm)	with 561nm laser ON
Pick only one fluorochrome from each row. *the 488 and 561 lasers share FL1-FL5 detetors.
FL1	525 BP 40	FITC, GFP, CFSE, Alexa 488, Calcein, DyLight 488, Live/Dead Green	-
FL2	575 BP 30	PE	Alexa 546, eFluor 585 NC, dsRed, tdTomato
FL3	620 BP 30	PE-Texas Red, ECD, PI	mCherry, Alexa 568
FL4	695 BP 30*	7-AAD, PerCP, PerCP-Cy5.5, PE-Cy5, PE-Cy5.5, PerCP-eFluor710	(675/30): mPlum
FL5	775 LP	PE-Cy7	-
FL6	660 BP 20	APC, Alexa 633, Alexa 647, eFluor 660, Live/Dead Far Red
FL7	725 BP 20	APC-Alexa 700
FL8	755 LP	APC-Cy7, APC-H7, APC-Alexa750, APC-eFluor780
FL9	450 BP 50	Pacific Blue, Alexa 405, Creulean, BV421, Horizon V450, DAPI, Hoechst, eFluor 450
FL10	550 BP 40	Krome Orange, FluoroGold, Pacific Orange, Alexa 430, BV 570, Cascade Yellow, Live/Dead Aqua
* 695 BP 30 is the standard filter and can be swapped for 675 BP 30 to maximise signal from certain fluorochromes
This list is an example of commonly used fluorochromes. For reagents not on the list, facility staff can help determine compatability.

Fortessa fluorochrome options.pdf	Download
Fortessa standard configuration.pdf (updated April 2020)	Download
CCB Fortessa guide.pdf	Download
Fortessa panel builder (std config).xlsx	Download

Laser	Filter
355 nm 20 mW	379/28
	515/30
	740/35
405 nm 50 mW	450/50
	525/50
	610/20
	660/20
	710/50
	780/60
488 nm 50 mW	488/10 (SSC)
	530/30
	710/50
561 nm 50 mW	585/15
	610/20
	670/30
	780/60
640 nm 40 mW	670/14
	730/45
	780/60

Astrios panel builder	astrios panel builder.xlsx
Astrios complete detector layout	Detector setup.pdf
Spare filters	448/59, 513/26, 546/20, 610/20, 614/20, 620/29, 664/22, 670/30 \| 600 LP, 650 LP

Our facility provides access to flow cytometers, cell-sorting and microscopes to assist users in achieving the best results for their studies.

Click on an instrument below for more information.